Regeneration is a complex and dynamic process, mobilizing diverse cell types and remodelling tissues over long time periods. Tracking cell fate and behaviour during regeneration in active adult animals is especially challenging. Here, we establish continuous live imaging of leg regeneration at single-cell resolution in the crustacean Parhyale hawaiensis. By live recordings encompassing the first 4-5 days after amputation, we capture the cellular events that contribute to wound closure and morphogenesis of regenerating legs with unprecedented resolution and temporal detail.
Biology is the natural science that involves the study of life and living organisms, including their physical and chemical structure, function, development and evolution. Modern biology is a vast field, composed of many branches. Despite the broad scope and the complexity of the science, there are certain unifying concepts that consolidate it into a single, coherent field. In general, biology recognizes the cell as the basic unit of life, genes as the basic unit of heredity, and evolution as the engine that propels the creation of new species. It is also understood that all organisms survive by consuming and transforming energy and by regulating their internal environment.